First Isolation and Identification of Agriphages in Vegetable Crops in West Africa (Côte d’Ivore): Potential Uses of Biocontrol in Plants

Agriphages or bacterial viruses are ubiquitous in the environment. The discovery of virulent phages against phytobacteria improves crop growth and proposes biopesticide uses for plant diseases. In Africa, many like Ralstonia, Clavibacter, Xanthomonas were reported several regions. study aims to evaluate presence agriphages biocontrol Côte d'Ivoire. Leaves soil samples collected from healthy diseased plants, three sites located Anyama, Abidjan, Bingerville. pretreatments occur with sterile physiological water leaves respectively. isolation was done on specific media campestris as host. Lytic activity tested agar five bacteria strains. After DNA extraction using Qiagen method kit, molecular confirmation by Random Amplified Polymorphic DNA-PCR. From this study, (5) isolated site 2. These have all been a broad spectrum lytic activity. Molecular characterization RAPD-PCR showed that these phages. dendrogram ΦXanS1, ΦXanS2 93% similarities. While ΦXanS1 62% similar ΦXanF1. This is first West Africa their potential infection crops.